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Los Lagos Region is one of Chile's 16 administrative regions and comprises four provinces: Chiloé, Llanquihue, Osorno and Palena. The region, located in Zona Sur, is known for its many lakes, Andean mountains, volcanoes and wild forest environments.

Stretching across Brazil, Bolivia and Paraguay, the Pantanal is a natural region encompassing the world's largest tropical wetland area. Although not as well known as the Amazon Rainforest to its north, this gigantic seasonal floodplain is also home to a staggering variety of plants and wildlife.

The Sierras Pampeanas (also called Central Sierras) is a geographical region and chain of mountains that rise sharply from the surrounding Pampas region of northwestern Argentina. They run parallel to the Andes, cross into seven Argentine provinces, and may be cataloged into sub-regions.

The Uruguayan savanna is a subtropical grassland and savanna ecoregion that extends from the extreme southern part of the Rio Grande do Sul, a Brazilian state, to include the entire country of Uruguay and a small section of the Argentinean province of Entre Ríos.

The Argentine Espinal ecoregion is a thorny deciduous shrubland forest, located in central Argentina, extending from central Santa Fe through the province of Córdoba to the north of San Luis. It has been extensively modified by large scale cattle ranching.

The Petén Basin is a geographical/archaeological subregion of Mesoamerica and a center of Maya civilizations such as Tikal and Calakmul. It is primarily located in northern Guatemala and southeastern Mexico. The Maya Forest is the largest remaining tropical rain forest in the Americas

The Humid Pampas occupy the plains in the east of Argentina and as well as one of the highest human-populated areas in Argentina, taking up most of the province of Buenos Aires. Horizontal plains and very soft undulations with low peaks that emerge like islands characterize the relief of this area.

The Semiarid Pampas ecoregion is located in the center of Argentina. The predominant vegetation consists of grasslands with some shrubs. There are few endemic plants in the ecoregion but the area has a high biodiversity.

The Atlantic Forest, or Mata Atlântica, extends along the Atlantic coast of northeast Brazil, south along the coastline and inland into northeast Argentina and eastern Paraguay. Although only a small part of the original forests remain it is still one of the most diverse ecosystems on the planet.

La Gran Sabana is a region in southeastern Venezuela that offers one of the most unusual landscapes in the world, with rivers, waterfalls and gorges, deep and vast valleys, as well as impenetrable jungles and savannas. The region also hosts the isolated tabletop mesas locally known as tepuis.