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Tak'alik Ab'aj is a pre-Columbian archaeological site located in southwestern Guatemala. The city was a commercial hub between the Guatemalan highlands and the Pacific coast. It is is one of several Mesoamerican sites with both Olmec and Maya features.

The Kanuku Mountains are a mountain group located within the Rupununi natural region in southwestern Guyana. The east-west range divides the Rupununi from the wetlands of the north and the savannas of the south. The mountains have been designated a National Protected Area.

The Rupununi is a natural region in southwest Guyana, bordering the Brazilian Amazon. It is divided into the northern and southern Rupununi by the Kanuku Mountains. The Rapununi River flows through the region before joining with the Essequibo River on its way to the Atlantic Ocean.

The Northeast Tobago Biosphere Reserve is located within the northeast region of the island of Tobago, made up of a large marine and terrestrial area. The reserve presents a rare Caribbean Island Ridge-to-Ocean ecosystem that includes the world's oldest tropical rain forest reserve.

The Essequibo River is the longest and widest river in Guyana. The river flows north through forest and savanna, including one of the largest continuous tracts of relatively pristine lowland tropical rain forest in the world, before emptying into the Atlantic Ocean.

Bahuaja-Sonene National Park of Peru is part of the largest multinational tropical protected area in the world. It protects an area comprised of tropical rain forest, savanna, flooded grasslands, hills and mountains; harboring some of the wildest and least impacted habitats in the world.

Cordillera Occidental

The Cordillera Occidental or Western Cordillera of Bolivia is a mountain range situated in extreme western Bolivia, forming a natural border with Chile.

Beginning in the north at Juqhuri on the border with Peru and ending in the south at the Licancabur volcano, the extent of the Cordillera Occidental is approximately 700 km (435 mi).

The Cordillera Oriental of Bolivia is part of the Andes Mountains system. It is the easternmost of Bolivia's two main ranges and separates the lowlands of the Amazon river basin to the east from the Altiplano to the west. The northern section of the range is known as the Cordillera Real.

The Bosques de Neblina - Selva Central Biosphere Reserve is located in the Amazon basin of central Peru, in a transition zone between the eastern slopes of the Andes and the Amazon. Harboring four emblematic Peruvian ecosystems, it is home to a high level of biological and cultural diversity.

Tambopata National Reserve of southeastern Peru is a massive subtropical rain forest reserve with forested hills, plains, swamps, oxbow lakes and meandering rivers. Its great wildlife diversity is due to its location at the confluence of lowland Amazon forest with three other ecosystems.